PROMO: α-Synuclein Assay Kits & Antibodies

background

Dementia is a disease that causes memory and loss of judgment. There is no basic treatment for people who develop the clinical picture. Years of scientific research have proven that protein aggregation occurs in many neurodegenerative diseases, including dementia. This shows that the occurrence of this aggregation is closely linked to diseases, development and disease progression. Aggregation occurs when normal protein forms abnormal structures for certain reasons and accumulates in the cell, with each disease having a different aggregating protein. It is known that the protein called “α-synuclein” forms abnormal structures in Parkinson’s, Lewy body dementia and multiple system atrophy in the patient’s brain.

The α-synuclein aggregation assay kit mimics the intracellular aggregation of “α-synuclein”, which enables in vitro screening of drugs.

α-Synuclein Kit composition

Componentsvolumeamountstorage4 ° Cdo not freeze
pCMV-SNCA (α – Synuclein Expression plasmid vector, Red cap) (conc. 1.25 μg / μL)32 μL1 vial
pCMV-NC (Negative control vector, Green cap)(conc. 1.25 μg / μL)5 μL1 vial
pCMV-dGFP (dGFP Expression plasmid vector, blue cap)(conc. 1.25 μg / μL)5 μL1 vial
20 mM Tris-HCl buffer (pH7.4)10 mL1 bottle
F-αSyn (α – Synuclein Fibrils, Yellow cap) (conc. 1 μg / μL)32 μL1 vial
Multifectam (gene transfection reagent)0.33 mg1 bottle

Products

α-Synuclein Aggregation Assay Kits

descriptionCatalog No.amount
α-Synuclein Aggregation Assay KitCSR-SYN01-COS1 KIT (300 tests)
Amyloid fluorescent staining kitCSR-SYN02-COS1 KIT (100 tests)
α-synuclein fibrilsCSR-SYN03-COS0.1 MG
α-synuclein, recombinant E. coliCSR-SYN04-COS1 MG
α-synuclein, recombinant E. coliCSR-SYN04-COS0.1 MG

Alpha-synuclein, a 140-amino acid protein that is abundantly expressed in presynaptic terminals and as a component of intraneuronal or glial inclusions, which is one of Parkinson’s (PD), dementia with Lewy bodies (DLB) and multiple system atrophy (MSA) play a major role. Alpha-Synuclein is a natively unfolded protein, but fibrillation or conformational change of Alpha-Synuclein is central to the pathogenesis of Alpha-Synucleinopathies. The amino-terminal region of alpha-synuclein consists of seven imperfect repeats, each 11 amino acids long, with the consensus sequence KTKEGV. The repetitions partially overlap with a hydrophobic region (amino acids 61-95). The carboxy-terminal region (amino acids 96-140) is negatively charged.

The antibodies on offer are powerful tools for biochemical and IHC analyzes of neurodegenerative diseases and for evaluating conformational changes of alpha-synuclein.

descriptionCatalog No.volume
Anti (ALPHA) Synuclein (1-10)CAC-TIP-SN-P0150UL
Anti (ALPHA) Synuclein (11-20)CAC-TIP-SN-P0250UL
Anti (ALPHA) Synuclein (21-30)CAC-TIP-SN-P0350UL
Anti (ALPHA) Synuclein (31-40)CAC-TIP-SN-P0450UL
Anti (ALPHA) Synuclein (41-50)CAC-TIP-SN-P0550UL
Anti (ALPHA) Synuclein (51-60)CAC-TIP-SN-P0650UL
Anti (ALPHA) Synuclein (61-70)CAC-TIP-SN-P0750UL
Anti (ALPHA) Synuclein (75-91)CAC-TIP-SN-P0850UL
Anti (ALPHA) Synuclein (131-140)CAC-TIP-SN-P0950UL
Anti (ALPHA) Synuclein (9 antibodies)CAC-TIP-SN-SET9 * 10UL

credentials

  • Airi T. et al. J Biol Chem. 2016 Sep 2; 291 (36): 18675-88
  • Masami Masuda, et al. Inhibition of a-synuclein fibril assembly by small molecules: A FEBS Letters (2009) 583, 787-791. PMID 19183551
  • Motokuni Yonetani, et al. Conversion of wild-type alpha-synuclein into mutant-type fib A30P mutant. Journal of Biological Chemistry (2009) 284, 7940-7950. PMID 19164293
  • Nonaka T. et al. J Biol Chem. 2010 Nov 5; 285 (45): 34885-98