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0032 (0)16 41 44 07 |
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APO ACTIVE 3
Phycoerythrin (PE)
Antibody Specific Active Caspase 3 Detection Kit
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| Key Benefits: |
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Highly specific for active human and murine caspase 3.
Other assays require the utilization of peptide based (DEVD)
reagents that tend to cross-react with caspase 7 and
other caspases.
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Applications - Works with a flow cytometer,
flourescence microscope, or 96 well plate reader.
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Yields both quantitative and qualitative results. Gives
a strong positive signal.
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Can be used in conjunction with other antibodies or
stains.
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No need to make cell lysates or run western blots. Cells
can be fixed and analyzed later.
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Works with human, mouse and rat cell lines.
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| Assay Principle: |
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Cell Technology ’s APO-ACTIVE 3 PE Kit utilizes a rabbit
affinity purified polyclonal antibody raised against amino
acid 163-175 of murine caspase 3 (9). This neo epitope is
present on the p18 subunit of cleaved caspase 3 (9). Cells
undergoing apoptosis are fixed and permeablized prior to the
addition of the antibody. A secondary PE labeled goat anti
rabbit antibody is used to visualize the bound rabbit anti
caspase 3 polyclonal. PE labeling allows for use with a
variety of applications.
|
| References: |
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Slee, E. A., C. Adrain, and S. J. Martin. 1999. Serial
Killers: orderingcaspase activation events in apoptosis.
Cell Death and Differ. 6:1067-1074.
-
Walker, N. P., R. V. Talanian, K. D. Brady, L. C. Dang,
N. J. Bump, C. R.Ferenz, S. Franklin, T. Ghayur, M. C.
Hackett and L. D. Hammill. 1994. Crystal Structure of
the Cysteine Protease Interleukin-1ß-Converting Enzyme:
A(p20/p10)2
Homodimer. Cell 78:343-352.
-
Wilson, K. P., J. F. Black, J. A. Thomson, E. E. Kim, J.
P. Griffith, M. A.Navia, M. A. Murcko, S. P. Chambers,
R. A. Aldape, S. A. Raybuck, and D. J.Livingston. 1994.
Structure and mechanism of interleukin-1 beta converting
enzyme. Nature 370: 270-275.
-
Rotonda, J., D. W. Nicholson, K. M. Fazil, M. Gallant,
Y. Gareau, M. Labelle,E. P. Peterson, D. M. Rasper, R.
Ruel, J. P. Vaillancourt, N. A. Thornberry and J.W.
Becker. 1996. The three-dimensional structure of apopain/CPP32,
a key mediator of apoptosis. Nature Struct. Biol. 3(7):
619-625.
-
Kumar, S. 1999. Mechanisms mediating caspase activation
in cell death. Cell Death and Differ. 6: 1060-1066.
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Alnemri, E.S. et al (1996) Cell 87:171
-
Trends Biochem Sci 22,388 (1997)
-
Nature 376, 37 (1995).
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| Kit contents: |
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| The
following kits are available: |
Product
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Catalog No.
|
Size
(No. of Tests) |
Price (US$) |
| APO ACTIVE 3 PE
Antibody Specific Active Caspase 3 Detection Kit |
PAB200-1 |
25 |
€145 |
PAB200-2
|
100 |
€425 |
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(A)
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(B) |
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