Apo Active 3 Phycoerythrin

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APO ACTIVE 3 Phycoerythrin (PE) ™
Antibody Specific Active Caspase 3 Detection Kit

Key Benefits:

Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents that tend to cross-react with caspase 7 and other caspases.
Applications - Works with a flow cytometer, flourescence microscope, or 96 well plate reader.
Yields both quantitative and qualitative results. Gives a strong positive signal.
Can be used in conjunction with other antibodies or stains.
No need to make cell lysates or run western blots. Cells can be fixed and analyzed later.
Works with human, mouse and rat cell lines.

Assay Principle:

Cell Technology ’s APO-ACTIVE 3 PE Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary PE labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal. PE labeling allows for use with a variety of applications.

Kit contents:

1 vial of Rabbit anti cleaved caspase 3 affinity purified polyclonal antibody
1 vial of PE labeled Goat anti Rabbit affinity purified polyclonal antibody
1 vial of 10X fixative.

*The following kits are available*:
Product	Catalog No.	Size (No. of Tests)	Price (Eur)
APO ACTIVE 3 PE Antibody Specific Active Caspase 3 Detection Kit	PAB200-1	25	€145
	PAB200-2	100	€425

(A)

(B)

Jurkat Cells were stimulated with staurosporine (B) or DMSO (A) for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.