APO LOGIX Sulforhodamine Caspase Detection
Kit ™
Apoptosis detection and in situ labeling of active caspase in
live cells.
Key Benefits:
Non-cytotoxic assay arrests further apoptotic activity
via caspase inhibition.
Cell permeablity permits direct visualization of
cytosolic apoptotic events.
Apoptotic cell population does not diminish over time.
Add reagent directly to cells. No special buffer or
media needed. No preparation of cell lysates required.
Simple wash procedure.
Works in diverse cell lines: human, rodent, Drosophila.
Can be performed in conjunction with Annexin staining,
TUNEL, antibody staining, or with other APO LOGIX
reagents on the same population of cells.
Permits high through-put screening. Protocol can be
adapted for ex vivo as well as in situ experiments.
Applications - Works with fluorescence
microscope, 96-well fluorescence plate readers
Yields both quantitative and qualitative results. Gives
strong signal with little background noise.
Assay Principle
APO LOGIX SR kits contain a generic sulforhodamine labeled
caspase inhibitor (sulforhodamine-peptide-fluoromethyl
ketone). This reagent is cell permeable and is used on whole
cells to detect apoptosis. Apoptotic cells are detected by a
fluorescence plate reader or fluorescence microscope using
an excitation source at 550nm and measuring emission at
595nm. The assay takes about 1 hr to complete
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